A rapid sensitive, flow cytometry-based method for the detection of Plasmodium vivax-infected blood cells.
Malar J
; 13: 55, 2014 Feb 14.
Article
de En
| MEDLINE
| ID: mdl-24528780
ABSTRACT
BACKGROUND:
Plasmodium vivax preferentially infects Duffy-positive reticulocytes and infections typically have few parasite-infected cells in the peripheral circulation. These features complicate detection and quantification by flow cytometry (FC) using standard nucleic acid-based staining methods. A simple antibody-based FC method was developed for rapid parasite detection along with simultaneous detection of other parasite and erythrocyte markers.METHODS:
Clinical samples were collected from patients diagnosed with P. vivax at a district Malaria Clinic in Kanchanaburi, Thailand. One µL of infected blood was washed, fixed, stained with a Plasmodium pan-specific anti-PfBiP antibody conjugated with Alexa Fluor 660, and analysed by FC. Additional primary conjugated antibodies for stage-specific markers of P. vivax for late trophozoite-early schizonts (MSP1-Alexa Fluor 660), late-stage schizonts (DBP-Alexa Fluor 555), and sexual stages (Pvs16) were used to differentiate intra-erythrocytic developmental stages.RESULTS:
The percentages of P. vivax-infected cells determined by the FC method and manually by microscopic examination of Giemsa-stained thick blood smears were positively correlated by Spearman's rank correlation coefficient (R2=0.93843) from 0.001 to 1.00% P. vivax-infected reticulocytes.CONCLUSIONS:
The FC-based method is a simple, robust, and efficient method for detecting P. vivax-infected reticulocytes.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Plasmodium vivax
/
Cellules sanguines
/
Paludisme à Plasmodium vivax
/
Cytométrie en flux
Type d'étude:
Diagnostic_studies
/
Evaluation_studies
Limites:
Humans
Pays/Région comme sujet:
Asia
Langue:
En
Journal:
Malar J
Sujet du journal:
MEDICINA TROPICAL
Année:
2014
Type de document:
Article