External validation of a multiplex urinary protein panel for the detection of bladder cancer in a multicenter cohort.
Cancer Epidemiol Biomarkers Prev
; 23(9): 1804-12, 2014 Sep.
Article
de En
| MEDLINE
| ID: mdl-24920641
ABSTRACT
BACKGROUND:
Because of the faltering sensitivity and/or specificity, urine-based assays currently have a limited role in the management of patients with bladder cancer. The aim of this study was to externally validate our previously reported protein biomarker panel from multiple sites in the United States and Europe.METHODS:
This multicenter external validation study included a total of 320 subjects (bladder cancer = 183). The 10 biomarkers (IL8, MMP9, MMP10, SERPINA1, VEGFA, ANG, CA9, APOE, SDC1, and SERPINE1) were measured using commercial ELISA assays in an external laboratory. The diagnostic performance of the biomarker panel was assessed using receiver operator curves (ROC) and descriptive statistical values.RESULTS:
Utilizing the combination of all 10 biomarkers, the area under the ROC for the diagnostic panel was noted to be 0.847 (95% confidence interval, 0.796-0.899), outperforming any single biomarker. The multiplex assay at optimal cutoff value achieved an overall sensitivity of 0.79, specificity of 0.79, positive prediction value of 0.73, and negative prediction value of 0.84 for bladder cancer classification. Sensitivity values of the diagnostic panel for high-grade bladder cancer, low-grade bladder cancer, muscle invasive bladder cancer, and non-muscle invasive bladder cancer were 0.81, 0.90, 0.95, and 0.77, respectively.CONCLUSIONS:
Urinary levels of the biomarker panel enabled discrimination of patients with bladder cancer and controls, and the levels of biomarker subsets were associated with advancing tumor grade and stage. IMPACT If proven to be reliable, urinary diagnostic biomarker assays can detect bladder cancer in a timely manner such that the patient can expect improvements in overall survival and quality of life.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Protéinurie
/
Tumeurs de la vessie urinaire
/
Marqueurs biologiques tumoraux
Type d'étude:
Clinical_trials
/
Diagnostic_studies
/
Etiology_studies
/
Incidence_studies
/
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Aspects:
Patient_preference
Limites:
Adult
/
Aged
/
Aged80
/
Humans
/
Male
/
Middle aged
Pays/Région comme sujet:
America do norte
/
Europa
Langue:
En
Journal:
Cancer Epidemiol Biomarkers Prev
Sujet du journal:
BIOQUIMICA
/
EPIDEMIOLOGIA
/
NEOPLASIAS
Année:
2014
Type de document:
Article