Single-cell analysis reveals functionally distinct classes within the planarian stem cell compartment.
Cell Stem Cell
; 15(3): 326-339, 2014 Sep 04.
Article
de En
| MEDLINE
| ID: mdl-25017721
ABSTRACT
Planarians are flatworms capable of regenerating any missing body region. This capacity is mediated by neoblasts, a proliferative cell population that contains pluripotent stem cells. Although population-based studies have revealed many neoblast characteristics, whether functionally distinct classes exist within this population is unclear. Here, we used high-dimensional single-cell transcriptional profiling from over a thousand individual neoblasts to directly compare gene expression fingerprints during homeostasis and regeneration. We identified two prominent neoblast classes that we named ζ (zeta) and σ (sigma). Zeta-neoblasts encompass specified cells that give rise to an abundant postmitotic lineage, including epidermal cells, and are not required for regeneration. By contrast, sigma-neoblasts proliferate in response to injury, possess broad lineage capacity, and can give rise to zeta-neoblasts. These findings indicate that planarian neoblasts comprise two major and functionally distinct cellular compartments.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Planaires
/
Cellules souches
/
Compartimentation cellulaire
/
Analyse sur cellule unique
Type d'étude:
Prognostic_studies
Limites:
Animals
Langue:
En
Journal:
Cell Stem Cell
Année:
2014
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique