[Quality assessment of Liuwei Wuling tablet by HPLC fingerprint and quantitative analysis].
Zhongguo Zhong Yao Za Zhi
; 39(10): 1816-21, 2014 May.
Article
de Zh
| MEDLINE
| ID: mdl-25282888
ABSTRACT
Reversed phase high performance liquid chromatography with diode array detector was employed for simultaneous determination of six components and specific chromatograms analysis in Liuwei Wuling tablets with gradient elution of acetonitrile and water containing 0.1% phosphoric acid as mobile phase. The results showed that six components containing specnuezhenide, phillyrin, schisandrin, schisantherin A, schizandrin A and schizandrin B were separated well under the analytical condition. The average recoveries ranged from 98.96% to 100.5% with RSD less than 2. 0%. Twenty-five common peaks were selected as the specific chromatograms of Liuwei Wuling tablets with schisantherin A as the reference peak. Similarities calculated by cosine of angle, correlation coefficient and peak area ratio similarity (PAR) were all above 0. 95, indicating a good similarity between the reference and twenty batches of samples. Grubbs test and cluster analysis indicated that the established HPLC fingerprints and HPLC quantitative analysis can be used efficiently in the quality control of Liuwei Wuling tablets.
Recherche sur Google
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Médicaments issus de plantes chinoises
/
Chromatographie en phase liquide à haute performance
Type d'étude:
Evaluation_studies
Langue:
Zh
Journal:
Zhongguo Zhong Yao Za Zhi
Sujet du journal:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Année:
2014
Type de document:
Article