Host interactions of Chandipura virus matrix protein.
Acta Trop
; 149: 27-31, 2015 Sep.
Article
de En
| MEDLINE
| ID: mdl-25944354
ABSTRACT
The rhabdovirus matrix (M) protein is a multifunctional virion protein that plays major role in virus assembly and budding, virus-induced inhibition of host gene expression and cytopathic effects observed in infected cells. The myriad roles played by this protein in the virus biology make it a critical player in viral pathogenesis. Therefore, discerning the interactions of this protein with host can greatly facilitate our understanding of virus infections, ultimately leading to both improved therapeutics and insight into cellular processes. Chandipura virus (CHPV; Family Rhabdoviridae, Genus Vesiculovirus) is an emerging rhabdovirus responsible for several outbreaks of fatal encephalitis among children in India. The present study aims to screen the human fetal brain cDNA library for interactors of CHPV M protein using yeast two-hybrid system. Ten host protein interactors were identified, three of which were further validated by affinity pull down and protein interaction ELISA. The study identified novel human host interactors for CHPV which concurred with previously described associations in other human viruses.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Protéines de la matrice virale
/
Vesiculovirus
/
Interactions hôte-pathogène
Limites:
Humans
Pays/Région comme sujet:
Asia
Langue:
En
Journal:
Acta Trop
Année:
2015
Type de document:
Article
Pays d'affiliation:
Inde