Tumor stroma and chemokines control T-cell migration into melanoma following Temozolomide treatment.
Oncoimmunology
; 4(2): e978709, 2015 Feb.
Article
de En
| MEDLINE
| ID: mdl-25949877
ABSTRACT
The infiltration of T lymphocytes within tumors is associated with better outcomes in cancer patients, yet current understanding of factors that influence T-lymphocyte infiltration into tumors remains incomplete. In our study, Temozolomide (TMZ), a chemotherapeutic drug used to treat metastatic melanoma, induced T-cell infiltration into transplanted melanoma and into genitourinary (GU) tumors in mice developing spontaneous melanoma. In contrast, TMZ treatment did not increase T-cell infiltration into cutaneous tumors, despite similar increases in the expression of the (C-X-C) chemokines CXCL9 and CXCL10 in all sites after TMZ exposure. Our findings reveal that the matrix architecture of the GU tumor stroma, and its ability to present CXCL9 and CXCL10 after TMZ treatment played a key role in favouring T-cell infiltration. We subsequently demonstrate that modifications of these key elements by combined collagenase and TMZ treatment induced T-cell infiltration into skin tumors. T cells accumulating within GU tumors after TMZ treatment exhibited T helper type-1 effector and cytolytic functional phenotypes, which are important for control of tumor growth. Our findings highlight the importance of the interaction between tumor stroma and chemokines in influencing T-cell migration into tumors, thereby impacting immune control of tumor growth. This knowledge will aid the development of strategies to promote T-cell infiltration into cancerous lesions and has the potential to markedly improve treatment outcomes.
CTL, cytolytic T lymphocyte; CXCL, Chemokine (C-X-C motif) ligand; ECM, extracellular matrix; GU, genitourinary; GZB, Granzyme B; HSPG, heparan sulphate proteoglycan; IFNγ, interferon γ; TAF, tumor-associated fibroblast; TILs, tumor-infiltrating lymphocytes; TMZ, Temozolomide; Th, T helper; Treg, T regulatory; WT, wild-type; chemokines; temozolomide; tumor stroma; tumor-infiltrating lymphocytes
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Langue:
En
Journal:
Oncoimmunology
Année:
2015
Type de document:
Article