Assessing patient characteristics and radiation-induced non-targeted effects in vivo for high dose-rate (HDR) brachytherapy.
Int J Radiat Biol
; 91(10): 786-94, 2015.
Article
de En
| MEDLINE
| ID: mdl-26136084
ABSTRACT
PURPOSE:
To test whether blood, urine, and tissue based colony-forming assays are a useful clinical detection tool for assessing fractionated treatment responses and non-targeted radiation effects in bystander cells. MATERIALS ANDMETHODS:
To assess patients' responses to radiation treatments, blood serum, urine, and an esophagus explant-based in vivo colony-forming assay were used from oesophageal carcinoma patients. These patients underwent three fractions of high dose rate (HDR) intraluminal brachytherapy (ILBT).RESULTS:
Human keratinocyte reporters exposed to blood sera taken after the third fraction of brachytherapy had a significant increase in cloning efficiency compared to baseline samples (p < 0.001). Such results may suggest an induced radioresistance response in bystander cells. The data also revealed a clear inverse dose-rate effect during late treatment fractions for the blood sera data only. Patient characteristics such as gender had no statistically significant effect (p > 0.05). Large variability was observed among the patients' tissue samples, these colony-forming assays showed no significant changes throughout fractionated brachytherapy (p > 0.05).CONCLUSION:
Large inter-patient variability was found in the urine and tissue based assays, so these techniques were discontinued. However, the simple blood-based assay had much less variability. This technique may have future applications as a biological dosimeter to predict treatment outcome and assess non-targeted radiation effects.Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Curiethérapie
/
Fractionnement de la dose d'irradiation
/
Effet bystander
Type d'étude:
Prognostic_studies
Limites:
Aged
/
Female
/
Humans
/
Male
Langue:
En
Journal:
Int J Radiat Biol
Sujet du journal:
RADIOLOGIA
Année:
2015
Type de document:
Article
Pays d'affiliation:
Canada