Comparison of the artus HIV-1 QS-RGQ and VERSANT HIV-1 RNA 1.0 assays for quantitative detection of human immunodeficiency virus type 1 in plasma samples.
New Microbiol
; 38(3): 337-44, 2015 Jul.
Article
de En
| MEDLINE
| ID: mdl-26147142
ABSTRACT
Several integrated diagnostic platforms to quantify human immunodeficiency virus type-1 viremia have been developed in recent years. We evaluated the performances of the Artus HIV-1 QS-RGQ assay, using the complete QIAsymphony RGQ workflow. 192 clinical plasma specimens and external control panel samples were analyzed, using the Artus assay and the routine Siemens VERSANT HIV-1 RNA 1.0 assay. Three samples were excluded due to amplification inhibition. Among the remaining 189 specimens, 130 samples were detected as positive (above the limit of detection by both assays; median log10 difference 0.01) and 18 samples were detected as negative. Eight samples (4.2%), all slightly above the limit of detection of the Versant assay, were negative with the Artus assay. The remaining 33 samples (beside 3 negative by Artus assay) were positive by both assays, but below the limit of detection at least in one of them. Results from the external panel samples showed a mean Log10 variation of -0.18 and -0.45 for the Versant and the Artus assays, respectively. As both assays showed highly correlated results, the QIAsymphony RGQ system, using the Artus HIV-1 QS-RGQ assay, could be considered a potential platform for HIV-1 RNA quantification in plasma.
Mots clés
Recherche sur Google
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
ARN viral
/
Infections à VIH
/
Réaction de polymérisation en chaîne
/
VIH-1 (Virus de l'Immunodéficience Humaine de type 1)
Type d'étude:
Diagnostic_studies
Limites:
Humans
Langue:
En
Journal:
New Microbiol
Sujet du journal:
MICROBIOLOGIA
Année:
2015
Type de document:
Article