Control of oxygen release from peroxides using polymers.
J Mater Sci Mater Med
; 26(7): 207, 2015 Jul.
Article
de En
| MEDLINE
| ID: mdl-26155978
ABSTRACT
An important limitation in cell therapy for the regeneration of tissue is the initial lack of oxygen. After implantation of large 3D cell-seeded structures, cells die rather than contribute to tissue regenerating. Here we've tested oxygen-releasing materials to improve cell survival and growth after implantation. Calcium peroxide (CaO2) in a polymer matrix was used as source of oxygen. Two polymers were tested in order to slow down and extend the period of oxygen release, poly(D,L-lactic acid) and poly(lactic-co-glycolic acid). Compared to CaO2 particles, both releasing systems showed an initially higher and shorter oxygen release. Human mesenchymal stromal cells cultured on casted films of these oxygen-releasing composites required catalase to proliferate, indicating the production of cytotoxic hydrogen peroxide as intermediate. Poly(D,L-lactic acid) and poly(lactic-co-glycolic acid) are less suited for slowly oxygen-releasing materials. Catalase was able to reduce the cytotoxic effect of H2O2.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Oxygène
/
Peroxydes
/
Polymères
Langue:
En
Journal:
J Mater Sci Mater Med
Sujet du journal:
ENGENHARIA BIOMEDICA
Année:
2015
Type de document:
Article
Pays d'affiliation:
Pays-Bas