ER and Golgi stresses increase ER-Golgi SNARE Syntaxin5: Implications for organelle stress and ßAPP processing.
Neurosci Lett
; 604: 30-5, 2015 Sep 14.
Article
de En
| MEDLINE
| ID: mdl-26219982
ABSTRACT
Unresolved endoplasmic reticulum (ER) stress causes neuronal death and has been implicated in neurodegenerative conditions such as Alzheimer's disease (AD). However, the mechanisms by which stress signals propagate from the ER through the Golgi apparatus and their effects on the transport and processing of AD-related proteins, such as ß-amyloid precursor protein (ßAPP), are unknown. We recently found that in the NG108-15 cell line, ER stress upregulates ER-Golgi-soluble N-ethylmaleimide-sensitive factor-attachment protein receptors (ER-Golgi SNAREs) Syx5 and Bet1. In the present study, we examined the effects of apoptosis and ER stress inducers on the expression of ER-Golgi SNARE proteins and cell viability in a primary culture of rat hippocampal neurons. An apoptosis inducer significantly downregulated the expression of ER-Golgi SNARE Syx5. ER-stress inducers upregulated the expression of Syx5 isoforms and Bet1 proteins via de novo synthesis of their mRNA transcripts. Knockdown of Syx5 during apoptosis or ER stress induction enhanced vulnerability of neurons. Additionally, we examined the effects of Golgi stress on Syx5 expression and ßAPP processing. Golgi stress also induced upregulation of ER-Golgi SNARE Syx5, and concomitantly, suppressed amyloid-ß peptide secretion. These findings suggest that Syx5 is a potential stress responsive factor that participates in ßAPP processing and the survival pathways of neuronal cells.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Organites
/
Précurseur de la protéine bêta-amyloïde
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Réticulum endoplasmique
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Protéines Qa-SNARE
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Stress du réticulum endoplasmique
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Appareil de Golgi
/
Neurones
Limites:
Animals
Langue:
En
Journal:
Neurosci Lett
Année:
2015
Type de document:
Article