RTVP-1 regulates glioma cell migration and invasion via interaction with N-WASP and hnRNPK.
Oncotarget
; 6(23): 19826-40, 2015 Aug 14.
Article
de En
| MEDLINE
| ID: mdl-26305187
ABSTRACT
Glioblastoma (GBM) are characterized by increased invasion into the surrounding normal brain tissue. RTVP-1 is highly expressed in GBM and regulates the migration and invasion of glioma cells. To further study RTVP-1 effects we performed a pull-down assay using His-tagged RTVP-1 followed by mass spectrometry and found that RTVP-1 was associated with the actin polymerization regulator, N-WASP. This association was further validated by co-immunoprecipitation and FRET analysis. We found that RTVP-1 increased cell spreading, migration and invasion and these effects were at least partly mediated by N-WASP. Another protein which was found by the pull-down assay to interact with RTVP-1 is hnRNPK. This protein has been recently reported to associate with and to inhibit the effect of N-WASP on cell spreading. hnRNPK decreased cell migration, spreading and invasion in glioma cells. Using co-immunoprecipitation we validated the interactions of hnRNPK with N-WASP and RTVP-1 in glioma cells. In addition, we found that overexpression of RTVP-1 decreased the association of N-WASP and hnRNPK. In summary, we report that RTVP-1 regulates glioma cell spreading, migration and invasion and that these effects are mediated via interaction with N-WASP and by interfering with the inhibitory effect of hnRNPK on the function of this protein.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Ribonucléoprotéines
/
Cellules souches tumorales
/
Tumeurs du cerveau
/
Mouvement cellulaire
/
Glioblastome
/
Protéine neuronale du syndrome de Wiskott-Aldrich
/
Protéines tumorales
/
Protéines de tissu nerveux
Limites:
Humans
Langue:
En
Journal:
Oncotarget
Année:
2015
Type de document:
Article
Pays d'affiliation:
Israël