Monobody-mediated alteration of enzyme specificity.
Nat Chem Biol
; 11(10): 762-4, 2015 Oct.
Article
de En
| MEDLINE
| ID: mdl-26322825
ABSTRACT
Current methods for engineering enzymes modify enzymes themselves and require a detailed mechanistic understanding or a high-throughput assay. Here, we describe a new approach where catalytic properties are modulated with synthetic binding proteins, termed monobodies, directed to an unmodified enzyme. Using the example of a ß-galactosidase from Bacillus circulans, we efficiently identified monobodies that restricted its substrates for its transgalactosylation reaction and selectively enhanced the production of small oligosaccharide prebiotics.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Oligosaccharides
/
Bacillus
/
Ingénierie des protéines
/
Protéines de transport
/
Beta-Galactosidase
/
Prébiotiques
Langue:
En
Journal:
Nat Chem Biol
Sujet du journal:
BIOLOGIA
/
QUIMICA
Année:
2015
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique