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Monobody-mediated alteration of enzyme specificity.
Tanaka, Shun-Ichi; Takahashi, Tetsuya; Koide, Akiko; Ishihara, Satoru; Koikeda, Satoshi; Koide, Shohei.
Affiliation
  • Tanaka S; Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois, USA.
  • Takahashi T; Frontier Research Department, Gifu R&D Center, Amano Enzyme, Inc., Gifu, Japan.
  • Koide A; Frontier Research Department, Gifu R&D Center, Amano Enzyme, Inc., Gifu, Japan.
  • Ishihara S; Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois, USA.
  • Koikeda S; Frontier Research Department, Gifu R&D Center, Amano Enzyme, Inc., Gifu, Japan.
  • Koide S; Frontier Research Department, Gifu R&D Center, Amano Enzyme, Inc., Gifu, Japan.
Nat Chem Biol ; 11(10): 762-4, 2015 Oct.
Article de En | MEDLINE | ID: mdl-26322825
ABSTRACT
Current methods for engineering enzymes modify enzymes themselves and require a detailed mechanistic understanding or a high-throughput assay. Here, we describe a new approach where catalytic properties are modulated with synthetic binding proteins, termed monobodies, directed to an unmodified enzyme. Using the example of a ß-galactosidase from Bacillus circulans, we efficiently identified monobodies that restricted its substrates for its transgalactosylation reaction and selectively enhanced the production of small oligosaccharide prebiotics.
Sujet(s)

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Oligosaccharides / Bacillus / Ingénierie des protéines / Protéines de transport / Beta-Galactosidase / Prébiotiques Langue: En Journal: Nat Chem Biol Sujet du journal: BIOLOGIA / QUIMICA Année: 2015 Type de document: Article Pays d'affiliation: États-Unis d'Amérique

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Oligosaccharides / Bacillus / Ingénierie des protéines / Protéines de transport / Beta-Galactosidase / Prébiotiques Langue: En Journal: Nat Chem Biol Sujet du journal: BIOLOGIA / QUIMICA Année: 2015 Type de document: Article Pays d'affiliation: États-Unis d'Amérique