Pharmacokinetics and tissue distribution of Aucubin, Ajugol and Catalpol in rats using a validated simultaneous LC-ESI-MS/MS assay.
J Chromatogr B Analyt Technol Biomed Life Sci
; 1002: 245-53, 2015 Oct 01.
Article
de En
| MEDLINE
| ID: mdl-26342167
ABSTRACT
To enable an investigation of pharmacokinetics and tissue distribution of Aucubin, Ajugol and Catalpol in rats, a high-performance liquid chromatography-electro spray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) method was developed for the simultaneous quantitative determination of the three compounds. Biological samples were prepared by a simple protein precipitation with methanol (containing 0.05% formic acid). The analytes were separated by a C18 reversed phase column and detected with a triple quadrupole tandem mass spectrometer in the multiple-reaction monitoring (MRM) mode to monitor the precursor-to-product ion transitions of m/z 364.3â149.0 for Aucubin, m/z 366.5â151.1 for Ajugol, m/z 380.0â183.3 for Catalpol and m/z 530.3â183.1 for Picroside-II (IS) in positive ionization. Good linearity of each calibration curve was produced over the concentration range of 1-1000ng/mL. The lower limit of quantification (LLOQ) was 1ng/mL for the three analytes. This method was successfully applied to the pharmacokinetic and tissue distribution studies of Aucubin, Ajugol and Catalpol in rat. The current results revealed pharmacokinetic behaviors of the herb compound and provided novel evidence of the presence of Aucubin and Catalpol in rat brain. The acquired data would be helpful for the clinical application and further studies of Traditional Chinese Medicines (TCM) with active ingredients of Iridoid Glycosides.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Pyrannes
/
Chromatographie en phase liquide à haute performance
/
Spectrométrie de masse ESI
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Spectrométrie de masse en tandem
/
Glycosides d'iridoïdes
/
Glucosides d'iridoïdes
Limites:
Animals
Langue:
En
Journal:
J Chromatogr B Analyt Technol Biomed Life Sci
Sujet du journal:
ENGENHARIA BIOMEDICA
Année:
2015
Type de document:
Article