Genome-wide identification and expression analysis of WNK kinase gene family in rice.
Comput Biol Chem
; 59 Pt A: 56-66, 2015 Dec.
Article
de En
| MEDLINE
| ID: mdl-26414948
ABSTRACT
Eukaryotic protein kinases represent one of the largest gene families involved in diverse regulatory functions. WNK (With No Lysine) kinases are members of ser/thr protein kinase family, which lack conserved catalytic lysine (K) residue at protein kinase subdomain II and is replaced by either asparagine, serine or glycine residues. They are involved in regulation of flowering time, circadian rhythms and abiotic stresses in Arabidopsis thaliana. In the present study, we have identified 9 members of WNK in rice, showed resemblance to Arabidopsis and human WNK and clustered into five main clades phylogenetically. The predicted genes structure, bonafide conserved signature motif and domains strongly support their identity, as members of WNK kinase family. We have analyzed their chromosomal distribution, physio-chemical properties, subcellular localizations and cis-elements in the promoter regions in silico. Further, transcript analysis of OsWNK by qRT-PCR revealed their differential regulation in tissue specific and abiotic stresses libraries. In conclusion, the identification of nine OsWNK and transcript level expression pattern under abiotic stress using qRT-PCR in rice will significantly contribute towards the understanding of WNK genes in monocots and thus provide a set up for functional genomics studies of WNK protein kinases.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Oryza
/
Protein-Serine-Threonine Kinases
/
Génome végétal
/
Régulation de l'expression des gènes végétaux
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Analyse de profil d'expression de gènes
Type d'étude:
Diagnostic_studies
Langue:
En
Journal:
Comput Biol Chem
Sujet du journal:
BIOLOGIA
/
INFORMATICA MEDICA
/
QUIMICA
Année:
2015
Type de document:
Article
Pays d'affiliation:
Inde