Genetic variability and phylogeny of the 5' long terminal repeat from Brazilian bovine leukemia virus.
Genet Mol Res
; 14(4): 14530-8, 2015 Nov 19.
Article
de En
| MEDLINE
| ID: mdl-26600512
ABSTRACT
We conducted a phylogenetic analysis of 22 strains of bovine leukemia virus obtained by polymerase chain reaction to amplify a 582-base pair fragment of the transcriptional regulatory region 5' long terminal repeat (LTR). Twenty-two samples of proviral DNA from peripheral blood mononuclear cells containing bovine leukemia virus from naturally infected bovine from 4 distinct geographic regions in Brazil were investigated. The products obtained by polymerase chain reaction were subjected to direct sequencing and sequence alignment. Fragments of 422 nucleotides were obtained, located between positions -118 and +303 base pairs of the 5'LTR. These fragments corresponded to 80% of the LTR region and included 56% of sub-region U3, 100% of R, and 82.5% of U5. Phylogenetic analysis of these sequences showed a high conservation degree in the 5'LTR region, with 5 well defined groups. However, a hotspot occurrence in the R-U5 region was also observed, which contained 40% of all nucleotide variability observed.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Phylogenèse
/
Variation génétique
/
Virus de la leucémie bovine
/
Séquences répétées terminales
Limites:
Animals
Pays/Région comme sujet:
America do sul
/
Brasil
Langue:
En
Journal:
Genet Mol Res
Sujet du journal:
BIOLOGIA MOLECULAR
/
GENETICA
Année:
2015
Type de document:
Article
Pays d'affiliation:
Brésil