Somatic Embryogenesis in Lisianthus (Eustoma russellianum Griseb.).
Methods Mol Biol
; 1359: 359-70, 2016.
Article
de En
| MEDLINE
| ID: mdl-26619872
ABSTRACT
Somatic embryogenesis is, for the main floricultural crops, a promising system for commercial scale-up, providing cloned material to be traded as seedlings. Somatic embryos, having the contemporary presence of root apical meristem and shoot apical meristem, can be readily acclimatized. For Lisianthus it is possible to induce embryogenic callus from leaf fragments of selected genotypes and to obtain embryos either in agarized substrate or in liquid suspension culture. The production of somatic embryos in liquid medium is high and can be modulated in order to synchronize the cycle and the size of the neoformed structures. The possibility to use the liquid substrate with high propagation rates reduces labor costs and could support the costs of eventual automation. In this paper we report a stepwise protocol for somatic embryogenesis in the species Eustoma russellianum.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Racines de plante
/
Gentianaceae
/
Techniques de culture de tissus
/
Techniques d'embryogenèse somatique végétale
Langue:
En
Journal:
Methods Mol Biol
Sujet du journal:
BIOLOGIA MOLECULAR
Année:
2016
Type de document:
Article
Pays d'affiliation:
Italie