Presensitization to Ascaris antigens promotes induction of mite-specific IgE upon mite antigen inhalation in mice.
Allergol Int
; 65(1): 44-51, 2016 Jan.
Article
de En
| MEDLINE
| ID: mdl-26666495
ABSTRACT
BACKGROUND:
Patients with house dust mite (HDM) allergy or Ascariasis produce serum IgE specific to the antigens of HDM or nematode Ascaris, respectively. Although human IgE cross-reactivity has been reported between HDM and Ascaris antigens, it remains unclear whether it contributes to the pathogenesis of allergic diseases. We herein investigated the induction of cross-reactive antibodies and T cells in mice and effects of airway exposure to HDM antigens after preimmunization with Ascaris antigens.METHODS:
Mice were intraperitoneally immunized with HDM or Ascaris antigens with Alum, followed by the intranasal administration of HDM antigens. Serum antigen-specific IgE and IgG were measured by ELISA. Cytokine release in splenocytes from Ascaris-immunized mice upon in vitro restimulation with HDM antigens were measured by ELISA.RESULTS:
Immunization with Ascaris or HDM antigens induced cross-reactive IgG1. Splenocytes from Ascaris-immunized mice released IL-5 and IL-13 in response to the restimulation with HDM antigens. Subsequent airway exposure to HDM antigens promoted the induction of HDM-specific IgE and upregulation of HDM-specific IgG1 in Ascaris-immunized mice, whereas these responses were not detected or smaller without the Ascaris presensitization.CONCLUSIONS:
We demonstrated that the immunization of naïve mice with Ascaris antigens induced production of antibodies and differentiation of Th2 cells, which were cross-reactive to HDM antigens, and accelerated induction of serum HDM-specific IgE upon subsequent airway exposure to HDM antigens in mice. These results suggest that sensitization to HDM towards IgE-mediated allergic diseases is faster in individuals with a previous history of Ascaris infection than in those without presensitization to Ascaris.Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Ascaris
/
Immunoglobuline E
/
Immunisation
/
Antigènes de Dermatophagoides
/
Hypersensibilité
/
Antigènes d'helminthe
Type d'étude:
Prognostic_studies
Limites:
Animals
Langue:
En
Journal:
Allergol Int
Sujet du journal:
ALERGIA E IMUNOLOGIA
Année:
2016
Type de document:
Article
Pays d'affiliation:
Japon