hCLE/C14orf166, a cellular protein required for viral replication, is incorporated into influenza virus particles.
Sci Rep
; 6: 20744, 2016 Feb 11.
Article
de En
| MEDLINE
| ID: mdl-26864902
ABSTRACT
The influenza A virus polymerase associates with a number of cellular transcription-related factors, including the RNA polymerase II (RNAP II). We previously described that the cellular protein hCLE/C14orf166 interacts with and stimulates influenza virus polymerase as well as RNAP II activities. Here we show that, despite the considerable cellular shut-off observed in infected cells, which includes RNAP II degradation, hCLE protein levels increase throughout infection in a virus replication-dependent manner. Human and avian influenza viruses of various subtypes increase hCLE levels, but other RNA or DNA viruses do not. hCLE colocalises and interacts with viral ribonucleoproteins (vRNP) in the nucleus, as well as in the cytoplasm late in infection. Furthermore, biochemical analysis of purified virus particles and immunoelectron microscopy of infected cells show hCLE in virions, in close association with viral vRNP. These findings indicate that hCLE, a cellular protein important for viral replication, is one of the very few examples of transcription factors that are incorporated into particles of an RNA-containing virus.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Ribonucléoprotéines
/
Protéines virales
/
Virion
/
Transactivateurs
/
Sous-type H1N1 du virus de la grippe A
/
Sous-type H3N2 du virus de la grippe A
/
Sous-type H9N2 du virus de la grippe A
Limites:
Animals
/
Humans
Langue:
En
Journal:
Sci Rep
Année:
2016
Type de document:
Article
Pays d'affiliation:
Espagne