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Mass spectrometry imaging of rat brain lipid profile changes over time following traumatic brain injury.
Roux, Aurelie; Muller, Ludovic; Jackson, Shelley N; Post, Jeremy; Baldwin, Katherine; Hoffer, Barry; Balaban, Carey D; Barbacci, Damon; Schultz, J Albert; Gouty, Shawn; Cox, Brian M; Woods, Amina S.
Affiliation
  • Roux A; Structural Biology Unit, Integrative Neuroscience Branch, NIH/NIDA-IRP, Baltimore, MD 21224, United States.
  • Muller L; Structural Biology Unit, Integrative Neuroscience Branch, NIH/NIDA-IRP, Baltimore, MD 21224, United States.
  • Jackson SN; Structural Biology Unit, Integrative Neuroscience Branch, NIH/NIDA-IRP, Baltimore, MD 21224, United States.
  • Post J; Structural Biology Unit, Integrative Neuroscience Branch, NIH/NIDA-IRP, Baltimore, MD 21224, United States.
  • Baldwin K; Structural Biology Unit, Integrative Neuroscience Branch, NIH/NIDA-IRP, Baltimore, MD 21224, United States.
  • Hoffer B; University Hospitals of Cleveland, Cleveland, OH 44106, United States.
  • Balaban CD; Departments of Otolaryngology, Neurobiology, Communication Sciences & Disorders, and Bioengineering, University of Pittsburgh, Pittsburgh, PA 15213, United States.
  • Barbacci D; Ionwerks, Inc, Houston, TX 77002, United States.
  • Schultz JA; Ionwerks, Inc, Houston, TX 77002, United States.
  • Gouty S; Center for Neuroscience and Regenerative Medicine, Department of Pharmacology, Uniformed Services University, Bethesda, MD 20814, United States.
  • Cox BM; Center for Neuroscience and Regenerative Medicine, Department of Pharmacology, Uniformed Services University, Bethesda, MD 20814, United States.
  • Woods AS; Structural Biology Unit, Integrative Neuroscience Branch, NIH/NIDA-IRP, Baltimore, MD 21224, United States. Electronic address: awoods@intra.nida.nih.gov.
J Neurosci Methods ; 272: 19-32, 2016 10 15.
Article de En | MEDLINE | ID: mdl-26872743
ABSTRACT

BACKGROUND:

Mild traumatic brain injury (TBI) is a common public health issue that may contribute to chronic degenerative disorders. Membrane lipids play a key role in tissue responses to injury, both as cell signals and as components of membrane structure and cell signaling. This study demonstrates the ability of high resolution mass spectrometry imaging (MSI) to assess sequences of responses of lipid species in a rat controlled cortical impact model for concussion. NEW

METHOD:

A matrix of implanted silver nanoparticles was implanted superficially in brain sections for matrix-assisted laser desorption (MALDI) imaging of 50µm diameter microdomains across unfixed cryostat sections of rat brain. Ion-mobility time-of-flight MS was used to analyze and map changes over time in brain lipid composition in a rats after Controlled Cortical Impact (CCI) TBI.

RESULTS:

Brain MS images showed changes in sphingolipids near the CCI site, including increased ceramides and decreased sphingomyelins, accompanied by changes in glycerophospholipids and cholesterol derivatives. The kinetics differed for each lipid class; for example ceramides increased as early as 1 day after the injury whereas other lipids changes occurred between 3 and 7 days post injury. COMPARISON WITH EXISTING METHOD(S) Silver nanoparticles MALDI matrix is a sensitive new tool for revealing previously undetectable cellular injury response and remodeling in neural, glial and vascular structure of the brain.

CONCLUSIONS:

Lipid biochemical and structural changes after TBI could help highlighting molecules that can be used to determine the severity of such injuries as well as to evaluate the efficacy of potential treatments.
Sujet(s)
Mots clés

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Spectrométrie de masse / Encéphale / Lésions traumatiques de l'encéphale / Lipides Type d'étude: Prognostic_studies Limites: Animals Langue: En Journal: J Neurosci Methods Année: 2016 Type de document: Article Pays d'affiliation: États-Unis d'Amérique

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Spectrométrie de masse / Encéphale / Lésions traumatiques de l'encéphale / Lipides Type d'étude: Prognostic_studies Limites: Animals Langue: En Journal: J Neurosci Methods Année: 2016 Type de document: Article Pays d'affiliation: États-Unis d'Amérique