The Role of Physical Stabilization in Whole Blood Preservation.
Sci Rep
; 6: 21023, 2016 Feb 15.
Article
de En
| MEDLINE
| ID: mdl-26876805
ABSTRACT
The rapid degradation of blood ex vivo imposes logistical limitations on the utilization of blood-borne cells in medical diagnostics and scientific investigations. A fundamental but overlooked aspect in the storage of this fluid tissue is blood settling, which induces physical stress and compaction, aggregates blood cells, and causes collateral damage due to leukocyte activation. Here we show that the polymer Ficoll 70 kDa stabilized blood samples and prevented blood settling over the course of 72 hours, primarily by inhibiting depletion-mediated red blood cell aggregation. Physical stabilization decreased echinocyte formation, improved leukocyte viability, and inhibited the release of neutrophil elastase--a marker of neutrophil extracellular trap formation. In addition, Ficoll-stabilized blood was compatible with common leukocyte enrichment techniques including red blood cell lysis and immunomagnetic purification. This study showed for the first time that blood settling can be prevented using polymers and has implications in diagnostics.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Conservation de sang
/
Agrégation cellulaire
/
Ficoll
Limites:
Humans
Langue:
En
Journal:
Sci Rep
Année:
2016
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique