3D Raman imaging of systemic endothelial dysfunction in the murine model of metastatic breast cancer.
Anal Bioanal Chem
; 408(13): 3381-7, 2016 May.
Article
de En
| MEDLINE
| ID: mdl-26935932
ABSTRACT
It was recently reported in the murine model of metastatic breast cancer (4T1) that tumor progression and development of metastasis is associated with systemic endothelial dysfunction characterized by impaired nitric oxide (NO) production. Using Raman 3D confocal imaging with the analysis of the individual layers of the vascular wall combined with AFM endothelial surface imaging, we demonstrated that metastasis-induced systemic endothelial dysfunction resulted in distinct chemical changes in the endothelium of the aorta. These changes, manifested as a significant increase in the protein content (18%) and a slight decrease in the lipid content (4%), were limited to the endothelium and did not occur in the deeper layers of the vascular wall. The altered lipid to protein ratio in the endothelium, although more pronounced in the fixed vascular wall, was also observed in the freshly isolated unfixed vascular wall samples in the aqueous environment (12 and 7% change of protein and lipid content, respectively). Our results support the finding that the metastasis induces systemic endothelial dysfunction that may contribute to cancer progression.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Analyse spectrale Raman
/
Tumeurs du sein
/
Endothélium vasculaire
/
Modèles animaux de maladie humaine
/
Métastase tumorale
Type d'étude:
Prognostic_studies
Limites:
Animals
Langue:
En
Journal:
Anal Bioanal Chem
Année:
2016
Type de document:
Article
Pays d'affiliation:
Pologne