Postmortem muscle protein degradation in humans as a tool for PMI delimitation.
Int J Legal Med
; 130(6): 1547-1555, 2016 Nov.
Article
de En
| MEDLINE
| ID: mdl-26951243
ABSTRACT
Forensic estimation of time since death relies on diverse approaches, including measurement and comparison of environmental and body core temperature and analysis of insect colonization on a dead body. However, most of the applied methods have practical limitations or provide insufficient results under certain circumstances. Thus, new methods that can easily be implemented into forensic routine work are required to deliver more and discrete information about the postmortem interval (PMI). Following a previous work on skeletal muscle degradation in the porcine model, we analyzed human postmortem skeletal muscle samples of 40 forensic cases by Western blotting and casein zymography. Our results demonstrate predictable protein degradation processes in human muscle that are distinctly associated with temperature and the PMI. We provide information on promising degradation markers for certain periods of time postmortem, which can be useful tools for time since death delimitation. In addition, we discuss external influencing factors such as age, body mass index, sex, and cause of death that need to be considered in future routine application of the method in humans.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Modifications postmortem
/
Muscles squelettiques
/
Protéolyse
Type d'étude:
Prognostic_studies
Limites:
Adolescent
/
Adult
/
Aged
/
Aged80
/
Child
/
Child, preschool
/
Female
/
Humans
/
Male
/
Middle aged
Langue:
En
Journal:
Int J Legal Med
Sujet du journal:
JURISPRUDENCIA
Année:
2016
Type de document:
Article
Pays d'affiliation:
Autriche