Mapping and modeling of a strain-specific epitope in the Norwalk virus capsid inner shell.
Virology
; 492: 232-41, 2016 May.
Article
de En
| MEDLINE
| ID: mdl-26971245
ABSTRACT
Noroviruses are diverse positive-strand RNA viruses associated with acute gastroenteritis. Cross-reactive epitopes have been mapped primarily to conserved sequences in the capsid VP1 Shell (S) domain, and strain-specific epitopes to the highly variable Protruding (P) domain. In this work, we investigated a strain-specific linear epitope defined by MAb NV10 that was raised against prototype (Genogroup I.1) strain Norwalk virus (NV). Using peptide scanning and mutagenesis, the epitope was mapped to amino acids 21-32 (LVPEVNASDPLA) of the NV S domain, and its specificity was verified by epitope transfer and reactivity with a recombinant MAb NV10 single-chain variable fragment (scFv). Comparative structural modeling of the NV10 strain-specific and the broadly cross-reactive TV20 epitopes identified two internal non-overlapping sites in the NV shell, corresponding to variable and conserved amino acid sequences among strains, respectively. The S domain, like the P domain, contains strain-specific epitopes that contribute to the antigenic diversity among the noroviruses.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Virus de Norwalk
/
Protéines de capside
/
Anticorps à chaîne unique
/
Anticorps antiviraux
/
Épitopes
Type d'étude:
Prognostic_studies
Limites:
Animals
Langue:
En
Journal:
Virology
Année:
2016
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique