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Disposable orbitally shaken TubeSpin bioreactor 600 for Sf9 cell cultivation in suspension.
Monteil, Dominique T; Shen, Xiao; Tontodonati, Giulia; Baldi, Lucia; Hacker, David L; Wurm, Florian M.
Affiliation
  • Monteil DT; Laboratory of Cellular Biotechnology (LBTC), École Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.
  • Shen X; Laboratory of Cellular Biotechnology (LBTC), École Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.
  • Tontodonati G; Laboratory of Cellular Biotechnology (LBTC), École Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.
  • Baldi L; Laboratory of Cellular Biotechnology (LBTC), École Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.
  • Hacker DL; Laboratory of Cellular Biotechnology (LBTC), École Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland; Protein Expression Core Facility (PECF), École Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.
  • Wurm FM; Laboratory of Cellular Biotechnology (LBTC), École Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland. Electronic address: florian.wurm@exellgene.com.
Anal Biochem ; 505: 26-8, 2016 07 15.
Article de En | MEDLINE | ID: mdl-27130502
ABSTRACT
Disposable orbitally shaken TubeSpin bioreactor 600 tubes (TS600s) were recently developed for the bench-scale cultivation of animal cells in suspension. Here we compared batch cultures of Sf9 insect cells in TS600s, spinner flasks, and shake flasks. Superior cell growth was observed in TS600s and shake flasks as compared with spinner flasks, and more favorable oxygen-enriched cell culture conditions were observed in TS600s as compared with either spinner or shake flasks. The results demonstrated the suitability of TS600s as a disposable vessel for the cultivation of Sf9 cells in suspension.
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Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Bioréacteurs / Cellules Sf9 Limites: Animals Langue: En Journal: Anal Biochem Année: 2016 Type de document: Article Pays d'affiliation: Suisse
Texte intégral
Ajouter à My VHL
Imprimer
XML
PubMed Links
Recherche sur Google

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Bioréacteurs / Cellules Sf9 Limites: Animals Langue: En Journal: Anal Biochem Année: 2016 Type de document: Article Pays d'affiliation: Suisse