Toxicity of Mitoxantrone-loaded Superparamagnetic Iron Oxide Nanoparticles in a HT-29 Tumour Spheroid Model.
Anticancer Res
; 36(6): 3093-101, 2016 Jun.
Article
de En
| MEDLINE
| ID: mdl-27272833
ABSTRACT
BACKGROUND/AIM:
Cancer research is commonly carried out in two-dimensional (2D) cell cultures, which poorly reflect in vivo settings where the growing tumours are exposed to mechanical forces and biochemical gradients. In this study we established a HF-29 colon carcinoma tumor spheroid model to investigate the effect of free mitoxantrone (MTO) and its nanoparticle-bound form (SPION(MTO)) under 3D cell culture conditions. MATERIALS ANDMETHODS:
Tumour spheroids were generated by seeding HT-29 colon carcinoma cells on agarose-coated cell culture wells. Growth of the spheroids was monitored daily by transmission microscopy upon treatment with free MTO, SPION(MTO) or unloaded SPION. RESULTS ANDCONCLUSION:
Unloaded SPION did not affect the spheroid size compared to untreated controls, while both free MTO and SPION(MTO) inhibited growth of the spheroids in a dose- and time-dependent manner. In comparison to free MTO, the effect of SPION(MTO) on spheroid growth was slightly delayed. Further analyses are necessary to investigate if MTO infiltrates spheroids in its nanoparticle-bound form or whether it is released from SPION before infiltration.Mots clés
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Composés du fer III
/
Mitoxantrone
/
Nanoparticules de magnétite
/
Antinéoplasiques
Limites:
Humans
Langue:
En
Journal:
Anticancer Res
Année:
2016
Type de document:
Article
Pays d'affiliation:
Allemagne