Valorizing recycled paper sludge by a bioethanol production process with cellulase recycling.
Bioresour Technol
; 216: 637-44, 2016 Sep.
Article
de En
| MEDLINE
| ID: mdl-27289054
ABSTRACT
The feasibility of cellulase recycling in the scope of bioethanol production from recycled paper sludge (RPS), an inexpensive byproduct with around 39% of carbohydrates, is analyzed. RPS was easily converted and fermented by enzymes and cells, respectively. Final enzyme partition between solid and liquid phases was investigated, the solid-bound enzymes being efficiently recovered by alkaline washing. RPS hydrolysis and fermentation was conducted over four rounds, recycling the cellulases present in both fractions. A great overall enzyme stability was observed 71, 64 and 100% of the initial Cel7A, Cel7B and ß-glucosidase activities, respectively, were recovered. Even with only 30% of fresh enzymes added on the subsequent rounds, solid conversions of 92, 83 and 71% were achieved for the round 2, 3 and 4, respectively. This strategy enabled an enzyme saving around 53-60%, while can equally contribute to a 40% reduction in RPS disposal costs.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Protéines de Saccharomyces cerevisiae
/
Cellulases
/
Éthanol
Langue:
En
Journal:
Bioresour Technol
Sujet du journal:
ENGENHARIA BIOMEDICA
Année:
2016
Type de document:
Article
Pays d'affiliation:
Portugal