Nuclear myosin I regulates cell membrane tension.
Sci Rep
; 6: 30864, 2016 08 02.
Article
de En
| MEDLINE
| ID: mdl-27480647
ABSTRACT
Plasma membrane tension is an important feature that determines the cell shape and influences processes such as cell motility, spreading, endocytosis and exocytosis. Unconventional class 1 myosins are potent regulators of plasma membrane tension because they physically link the plasma membrane with adjacent cytoskeleton. We identified nuclear myosin 1 (NM1) - a putative nuclear isoform of myosin 1c (Myo1c) - as a new player in the field. Although having specific nuclear functions, NM1 localizes predominantly to the plasma membrane. Deletion of NM1 causes more than a 50% increase in the elasticity of the plasma membrane around the actin cytoskeleton as measured by atomic force microscopy. This higher elasticity of NM1 knock-out cells leads to 25% higher resistance to short-term hypotonic environment and rapid cell swelling. In contrast, overexpression of NM1 in wild type cells leads to an additional 30% reduction of their survival. We have shown that NM1 has a direct functional role in the cytoplasm as a dynamic linker between the cell membrane and the underlying cytoskeleton, regulating the degree of effective plasma membrane tension.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Peau
/
Cytosquelette d'actine
/
Membrane cellulaire
/
Noyau de la cellule
/
Myosine de type I
/
Fibroblastes
Limites:
Animals
/
Humans
Langue:
En
Journal:
Sci Rep
Année:
2016
Type de document:
Article
Pays d'affiliation:
République tchèque