Adhesion and Fusion of Muscle Cells Are Promoted by Filopodia.
Dev Cell
; 38(3): 291-304, 2016 08 08.
Article
de En
| MEDLINE
| ID: mdl-27505416
ABSTRACT
Indirect flight muscles (IFMs) in Drosophila are generated during pupariation by fusion of hundreds of myoblasts with larval muscle templates (myotubes). Live observation of these muscles during the fusion process revealed multiple long actin-based protrusions that emanate from the myotube surface and require Enabled and IRSp53 for their generation and maintenance. Fusion is blocked when formation of these filopodia is compromised. While filopodia are not required for the signaling process underlying critical myoblast cell-fate changes prior to fusion, myotube-myoblast adhesion appears to be filopodia dependent. Without filopodia, close apposition between the cell membranes is not achieved, the cell-adhesion molecule Duf is not recruited to the myotube surface, and adhesion-dependent actin foci do not form. We therefore propose that the filopodia are necessary to prime the heterotypic adhesion process between the two cell types, possibly by recruiting the cell-adhesion molecule Sns to discrete patches on the myoblast cell surface.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Pseudopodes
/
Adhérence cellulaire
/
Membrane cellulaire
/
Fibres musculaires squelettiques
/
Protéines de Drosophila
/
Myoblastes
/
Drosophila melanogaster
Limites:
Animals
Langue:
En
Journal:
Dev Cell
Sujet du journal:
EMBRIOLOGIA
Année:
2016
Type de document:
Article
Pays d'affiliation:
Israël