Simultaneous determination of individual isothiocyanates in plant samples by HPLC-DAD-MS following SPE and derivatization with N-acetyl-l-cysteine.
Food Chem
; 214: 587-596, 2017 Jan 01.
Article
de En
| MEDLINE
| ID: mdl-27507514
ABSTRACT
The procedure for the isothiocyanates (ITCs) determination that involves derivatization with N-acetyl-l-cysteine (NAC) and separation by HPLC was developed. Prior to derivatization, plant ITCs were isolated and purified using solid-phase extraction (SPE). The optimum conditions of derivatization are 500µL of isopropanolic eluate obtained by SPE combined with 500µL of derivatizing reagent (0.2M NAC and 0.2M NaHCO3 in water) and reaction time of 1h at 50°C. The formed dithiocarbamates are directly analyzed by HPLC coupled with diode array detector and mass spectrometer if required. The method was validated for nine common natural ITCs. Calibration curves were linear (R(2)⩾0.991) within a wide range of concentrations and limits of detection were below 4.9nmol/mL. The recoveries were in the range of 83.3-103.7%, with relative standard deviations <5.4%. The developed method has been successfully applied to determine ITCs in broccoli, white cabbage, garden cress, radish, horseradish and papaya.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Plantes
/
Acétylcystéine
/
Spectrométrie de masse
/
Chromatographie en phase liquide à haute performance
/
Chromatographie en phase liquide
/
Isothiocyanates
Langue:
En
Journal:
Food Chem
Année:
2017
Type de document:
Article
Pays d'affiliation:
Pologne