Combined IKZF1 and IG markers as new tools for diagnosis and minimal residual disease assessment in Tunisian B-ALL.
Bull Cancer
; 103(10): 822-828, 2016 Oct.
Article
de En
| MEDLINE
| ID: mdl-27614734
ABSTRACT
INTRODUCTION:
The monitoring of minimal residual disease (MRD) approach in patients diagnosed with B-acute lymphoblastic leukemia (B-ALL) allows an early detection of residual clones inducing relapses and therefore appropriate therapy strategy. The molecular markers may identify and quantify the residual blasts in B-ALL with normal cytology. In this study, we aimed to use combined IKZF1, IGH and IGK immunoglobulin genes for diagnosis and MRD monitoring in B-ALL sample using MLPA, multiplex PCR and real-time quantitative PCR.MATERIAL:
We showed that multiplex PCR and MLPA are necessary and complementary to detect IKZF1 deletions.RESULTS:
We have identified at the diagnosis clonal IGH rearrangement (VH3-JH5) and IKZF1 deletion (Δ4-7), which we have used it for MRD evaluation after induction chemotherapy. Despite the absence of chromosome abnormality, the patient may be classified in high-risk group with a relapse rate of residual blasts>10-4 and sensitivity up to 10-5. This molecular approach enabled the patient's stratification, which was overlooked by classical methods.CONCLUSION:
The combined IKZF1 and immunoglobulin genes will be used as appropriate molecular tools for diagnosis and MRD assessment of B-lineage leukemias and introduced as a routine tests in Tunisian clinical laboratories. They will be useful to stratify patients into risk groups leading to better treatment strategy.Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Gènes d'immunoglobuline
/
Leucémie-lymphome lymphoblastique à précurseurs B
/
Chaines lourdes des immunoglobulines
/
Chaines légères des immunoglobulines
/
Délétion de gène
/
Facteur de transcription Ikaros
Type d'étude:
Diagnostic_studies
/
Prognostic_studies
/
Screening_studies
Limites:
Humans
Langue:
En
Journal:
Bull Cancer
Année:
2016
Type de document:
Article
Pays d'affiliation:
Tunisie