Oxo-Functionalized Graphene as a Cell Membrane Carrier of Nucleic Acid Probes Controlled by Aging.
Chemistry
; 22(43): 15389-15395, 2016 Oct 17.
Article
de En
| MEDLINE
| ID: mdl-27619408
We applied a fluorescein-containing oligonucleotide (ON) to probe surface properties of oxidized graphene (oxo-G) and observed that graphene-like patches are formed upon aging of oxo-G, indicated by enhanced probe binding and by FTIR spectroscopic analysis. By using a recently developed fluorogenic endoperoxide (EP) probe, we confirmed that during the aging process the amount of EPs on the oxo-G surface is reduced. Furthermore, aging was found to strongly affect cell membrane carrier properties of this material. In particular, freshly prepared oxo-G does not act as a carrier, whereas oxo-G aged for 28â
days at 4 °C is an excellent carrier. Based on these data we prepared an optimized oxo-G, which has a low-defect density, binds ONs, is not toxic, and acts as cell membrane carrier. We successfully applied this material to design fluorogenic probes of representative intracellular nucleic acids 28S rRNA and ß-actin-mRNA. The results will help to standardize oxidized graphene derivatives for biomedical and bioanalytical applications.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Oligonucléotides
/
Sondes d'acide nucléique
/
Membrane cellulaire
/
Actines
/
Nanostructures
/
Graphite
Langue:
En
Journal:
Chemistry
Sujet du journal:
QUIMICA
Année:
2016
Type de document:
Article
Pays d'affiliation:
Allemagne
Pays de publication:
Allemagne