Dexmedetomidine protected COPD-induced lung injury by regulating miRNA-146a.
Bratisl Lek Listy
; 117(9): 539-542, 2016.
Article
de En
| MEDLINE
| ID: mdl-27677199
ABSTRACT
OBJECTIVE:
To study the mechanism of protection provided by dexmedetomidine against COPD-induced lung injury.METHODS:
COPD rat model was determined by measuring lung function, and comparing HE staining between two different groups. We got the lung tissue and cells from the control and COPD groups. The cells were divided into three groups control group, and blank and drug groups that were from the COPD rats. Cell apoptosis, relative gene expression and TNF-α and IL-1ß from nutrient solution were measured.RESULTS:
The TV, PEF, EF50, FEV0.3 and FEV0.3/FVC in COPD group were significantly lower than in control group (1.26±0.17 vs 2.65±0.21; 17.61±0.35 vs 38.55±0.24; 1.20±0.14 vs 1.81±0.06; 2.52±0.28 vs 4.44±0.26; 63.39±0.22 vs 88.45±0.34, p < 0.05, respectively). Cell apoptosis was significantly different in blank and drug groups (21.65±0.86 vs 10.74±0.15; p < 0.05, respectively). The gene expressions of miRNA-146a, p53 and Bcl-2 were significantly downregulated compared with blank group.CONCLUSION:
Dexmedetomidine protected COPD-induced lung injury by inhibiting miRNA-146a expression to reduce cell apoptosis (Tab. 1, Fig. 3, Ref. 25).Mots clés
Recherche sur Google
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Dexmédétomidine
/
Broncho-pneumopathie chronique obstructive
/
MicroARN
/
Lésion pulmonaire aigüe
/
Anti-inflammatoires
Type d'étude:
Prognostic_studies
Limites:
Animals
Langue:
En
Journal:
Bratisl Lek Listy
Année:
2016
Type de document:
Article