Single-step microfluidic synthesis of transferrin-conjugated lipid nanoparticles for siRNA delivery.
Nanomedicine
; 13(2): 371-381, 2017 02.
Article
de En
| MEDLINE
| ID: mdl-27720989
ABSTRACT
Microfluidic systems can accelerate clinical translation of nanoparticles due to their ability to generate nanoparticles in a well-controlled and reproducible manner. In this study, a single-step process based on microfluidic focusing (MF) was employed to synthesize transferrin-conjugated lipid nanoparticles (Tf-LNPs) and the method was compared with a multi-steps bulk mixing (BM) method. The results indicate that this single-step MF process enables rapid and efficient synthesis of Tf-LNPs, which were named Tf-LNPs-MF. Tf-LNPs-MF was shown to have a smaller size and more uniform structures compared to LNPs produced by multi-steps BM method (Tf-LNPs-BM). Furthermore, efficient cellular uptake of Tf-LNPs-MF in vitro as well as greater tumor inhibition in vivo proved that Tf-LNPs-MF had higher siRNA delivery efficiency in vitro and in vivo. Taken together, this single-step microfluidic synthesis significantly simplified the Tf-LNPs production and improved their drug delivery properties and may serve as a valuable tool for developing new cancer therapies.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Petit ARN interférent
/
Microfluidique
/
Nanoparticules
Limites:
Humans
Langue:
En
Journal:
Nanomedicine
Sujet du journal:
BIOTECNOLOGIA
Année:
2017
Type de document:
Article
Pays d'affiliation:
Chine