Genetic code expansion for multiprotein complex engineering.
Nat Methods
; 13(12): 997-1000, 2016 Dec.
Article
de En
| MEDLINE
| ID: mdl-27749839
ABSTRACT
We present a baculovirus-based protein engineering method that enables site-specific introduction of unique functionalities in a eukaryotic protein complex recombinantly produced in insect cells. We demonstrate the versatility of this efficient and robust protein production platform, 'MultiBacTAG', (i) for the fluorescent labeling of target proteins and biologics using click chemistries, (ii) for glycoengineering of antibodies, and (iii) for structure-function studies of novel eukaryotic complexes using single-molecule Förster resonance energy transfer as well as site-specific crosslinking strategies.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Protéines virales
/
Protéines recombinantes
/
Ingénierie des protéines
/
Complexes multiprotéiques
/
Protéines à fluorescence verte
Limites:
Animals
/
Humans
Langue:
En
Journal:
Nat Methods
Sujet du journal:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Année:
2016
Type de document:
Article
Pays d'affiliation:
Allemagne