Genetically edited pigs lacking CD163 show no resistance following infection with the African swine fever virus isolate, Georgia 2007/1.
Virology
; 501: 102-106, 2017 01 15.
Article
de En
| MEDLINE
| ID: mdl-27898335
ABSTRACT
African swine fever is a highly contagious, often fatal disease of swine for which there is no vaccine or other curative treatment. The macrophage marker, CD163, is a putative receptor for African swine fever virus (ASFV). Pigs possessing a complete knockout of CD163 on macrophages were inoculated with Georgia 2007/1, a genotype 2 isolate. Knockout and wild type pen mates became infected and showed no differences in clinical signs, mortality, pathology or viremia. There was also no difference following in vitro infection of macrophages. The results do not rule out the possibility that other ASFV strains utilize CD163, but demonstrate that CD163 is not necessary for infection with the Georgia 2007/1 isolate. This work rules out a significant role for CD163 in ASFV infection and creates opportunities to focus on alternative receptors and entry mechanisms.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Suidae
/
Animal génétiquement modifié
/
Récepteurs de surface cellulaire
/
Peste porcine africaine
/
Virus de la peste porcine africaine
Limites:
Animals
Pays/Région comme sujet:
America do norte
Langue:
En
Journal:
Virology
Année:
2017
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique