Dynamic Phenotypic Transition of Breast Cancer Cells In Vitro Revealed by Self-floating Cell Culture.
Anticancer Res
; 37(4): 1793-1797, 2017 04.
Article
de En
| MEDLINE
| ID: mdl-28373443
ABSTRACT
BACKGROUND:
Breast tumor heterogeneity leads to phenotypic diversity, such as tumor-initiating and metastatic properties and drug sensitivity. MATERIALS ANDMETHODS:
We found that a self-floating cell (SFC) culture enriches a drug-resistant subpopulation in a HER2-positive breast cancer cell line. SFCs were analyzed for cancer stem cell markers, gene expression profiles, and sensitivity for anticancer drugs.RESULTS:
SFCs expressed cancer stem cell markers, such as aldehyde dehydrogenase (ALDH) activity and elevated HER2 autophosphorylation. Gene expression profiles of SFCs showed a dramatic difference compared to those of parental or forced floating cells. SFCs also expressed CD133, a marker of drug resistance, and resisted cytotoxic drugs by drug efflux transporters. In contrast, HER2 kinase inhibitors efficiently reduced SFC viability.CONCLUSION:
SFCs enrich drug-resistant subpopulations even in vitro and might reflect the highly plastic nature of breast cancer cells even in vitro.Mots clés
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Cellules souches tumorales
/
Tumeurs du sein
/
Adénocarcinome
/
Adénocarcinome papillaire
/
Techniques de culture cellulaire
Limites:
Female
/
Humans
Langue:
En
Journal:
Anticancer Res
Année:
2017
Type de document:
Article
Pays d'affiliation:
Japon