Long non-coding RNA MEG3 inhibits adipogenesis and promotes osteogenesis of human adipose-derived mesenchymal stem cells via miR-140-5p.
Mol Cell Biochem
; 433(1-2): 51-60, 2017 Sep.
Article
de En
| MEDLINE
| ID: mdl-28382492
ABSTRACT
lncRNAs are an emerging class of regulators involved in multiple biological processes. MEG3, an lncRNA, acts as a tumor suppressor, has been reported to be linked with osteogenic differentiation of MSCs. However, limited knowledge is available concerning the roles of MEG3 in the multilineage differentiation of hASCs. The current study demonstrated that MEG3 was downregulated during adipogenesis and upregulated during osteogenesis of hASCs. Further functional analysis showed that knockdown of MEG3 promoted adipogenic differentiation, whereas inhibited osteogenic differentiation of hASCs. Mechanically, MEG3 may execute its role via regulating miR-140-5p. Moreover, miR-140-5p was upregulated during adipogenesis and downregulated during osteogenesis in hASCs, which was negatively correlated with MEG3. In conclusion, MEG3 participated in the balance of adipogenic and osteogenic differentiation of hASCs, and the mechanism may be through regulating miR-140-5p.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Ostéogenèse
/
Différenciation cellulaire
/
Tissu adipeux
/
MicroARN
/
Adipogenèse
/
Cellules souches mésenchymateuses
/
ARN long non codant
Limites:
Humans
Langue:
En
Journal:
Mol Cell Biochem
Année:
2017
Type de document:
Article
Pays d'affiliation:
Chine