Heterodimeric V. nikolskii phospholipases A2 induce aggregation of the lipid bilayer.
Toxicon
; 133: 169-179, 2017 Jul.
Article
de En
| MEDLINE
| ID: mdl-28528175
ABSTRACT
We report that the action of the heterodimeric phospholipases A2 (PLA2s) from Vipera nikolskii, which comprises enzymatically active basic subunit and inactive acidic PLA2 homologue, on the lipid bilayer results in the aggregation and stacking of bilayers. These processes are demonstrated using two independent methods (fluorescence spectroscopy and electron microscopy). Aggregation of bilayers is possible because both subunits of the V. nikolskii heterodimer contain a membrane-binding site (also known as IBS). Thus, when the two IBSs bind to the membrane, the heterodimer acts as a connecting agent. Heterodimers induce aggregation of negatively charged bilayers composed of phosphatidylglycerol and do not induce aggregation of neutral bilayers composed of phosphatidylcholine.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Venins de vipère
/
Viperidae
/
Phospholipases A2
/
Double couche lipidique
Limites:
Animals
Langue:
En
Journal:
Toxicon
Année:
2017
Type de document:
Article
Pays d'affiliation:
Russie