Enhanced LC-MS/MS analysis of alogliptin and pioglitazone in human plasma: Applied to a preliminary pharmacokinetic study.
J Chromatogr B Analyt Technol Biomed Life Sci
; 1058: 93-101, 2017 Jul 15.
Article
de En
| MEDLINE
| ID: mdl-28528664
ABSTRACT
A new fast LC-MS/MS method was developed for determination of alogliptin and pioglitazone in human plasma. Linearity ranges of 10-400ngmL-1 for alogliptin and 25-2000ngmL-1 for pioglitazone, were found to be suitable for their bioanalysis covering the Cmin and Cmax values of the drugs. Direct precipitation technique was used for simultaneous extraction of the drugs successfully from human plasma samples. Chromatographic separation was achieved on a BEH C18 column (50mm×2.1mm, 1.7µm) with 0.1% aqueous formic acid acetonitrile (4060, v/v) at a flow rate of 0.3mLmin-1. The validated method was applied to a preliminary pharmacokinetic study on human volunteers. Monitoring the transition pairs of m/z 340.18 to 116.08 for alogliptin and m/z 356.99 to 133.92 for pioglitazone, using triple quadrupole mass spectrometer with multiple reaction monitoring, was achieved in the positive mode. The validated method is accurate and suitable for further clinical applications and possible bioequivalence studies.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Pipéridines
/
Uracile
/
Chromatographie en phase liquide
/
Thiazolidinediones
/
Spectrométrie de masse en tandem
Type d'étude:
Diagnostic_studies
/
Prognostic_studies
Limites:
Adult
/
Humans
Langue:
En
Journal:
J Chromatogr B Analyt Technol Biomed Life Sci
Sujet du journal:
ENGENHARIA BIOMEDICA
Année:
2017
Type de document:
Article
Pays d'affiliation:
Égypte