Regulation of miRNA-146a and miRNA-150 Levels by Celecoxib in Premalignant Lesions of K14-HPV16 Mice.
Anticancer Res
; 37(6): 2913-2918, 2017 06.
Article
de En
| MEDLINE
| ID: mdl-28551628
ABSTRACT
BACKGROUND/AIM:
Human papillomavirus type 16 (HPV16) induces various types of cancer in several locations. Microenvironmental microRNAs (miRNAs) such as miRNA-146a and miRNA-150 regulate cancer-associated inflammation and are involved in HPV-induced carcinogenesis. We studied the effects of celecoxib on the expression of these two miRNAs in HPV16-induced lesions. MATERIALS ANDMETHODS:
Female transgenic (HPV16+/-) and wild-type (HPV16-/-) mice were administered 75 mg/kg/day celecoxib orally (treatment groups) or placebo (control groups) for four weeks. Skin samples were classified histologically, or used for miRNA analysis by quantitative real-time PCR.RESULTS:
HPV16+/- mice showed higher miRNA-146a and miRNA-150 expression levels compared to wild-type animals. Celecoxib further increased miRNA-150 (p<0.05) and miRNA-146a levels in treated animals. Celecoxib-treated HPV16+/- animals also showed reduced incidence of epidermal dysplasia and reduced inflammation, compared to untreated mice.CONCLUSION:
In this model, celecoxib may be able to regulate tumour-associated inflammation, through mechanisms involving the regulation of miRNA-146a and miRNA-150.Mots clés
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
États précancéreux
/
MicroARN
/
Papillomavirus humain de type 16
/
Inhibiteurs de la cyclooxygénase 2
/
Célécoxib
Limites:
Animals
Langue:
En
Journal:
Anticancer Res
Année:
2017
Type de document:
Article
Pays d'affiliation:
Portugal