Autophosphorylation Assays Using Plant Receptor Kinases Synthesized in Cell-Free Systems.
Methods Mol Biol
; 1621: 113-120, 2017.
Article
de En
| MEDLINE
| ID: mdl-28567648
ABSTRACT
The wheat germ cell-free protein synthesis system has a significant advantage for high-throughput production of eukaryotic multidomain proteins in a folded state. In this chapter, we describe two kinds of methods for performing autophosphorylation assay of plant receptor kinases (PRKs) by using the wheat cell-free system. One is an in vitro kinase assay performed using biotin-streptavidin affinity purification technology, and the other is a luminescence-based high-throughput assay for autophosphorylation analysis. We anticipate that our cell-free-based methods might facilitate the characterization of plant PRKs.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Triticum
/
Système acellulaire
/
Maturation post-traductionnelle des protéines
/
Arabidopsis
/
Récepteurs de surface cellulaire
/
Protéines d'Arabidopsis
/
Mesures de luminescence
Langue:
En
Journal:
Methods Mol Biol
Sujet du journal:
BIOLOGIA MOLECULAR
Année:
2017
Type de document:
Article
Pays d'affiliation:
Japon