Detection of canine adenovirus 1 in red foxes ( Vulpes vulpes) and raccoons ( Procyon lotor) in Germany with a TaqMan real-time PCR assay.
J Vet Diagn Invest
; 29(5): 741-746, 2017 Sep.
Article
de En
| MEDLINE
| ID: mdl-28581363
ABSTRACT
We developed a real-time (rt)PCR assay based on TaqMan probe technology for the specific detection of canine adenovirus 1 (CAdV-1). The assay is able to detect three 50% tissue culture infectious dose/mL in CAdV-1-containing cell culture supernatant. Viral genomes were not amplified of canine adenovirus 2 or of several bovine, porcine, and avian adenoviruses. In silico analysis provided no indication of amplification of other heterologous genomes. The sensitivity of the real-time assay exceeded that of a conventional gel-based CAdV-1 PCR by a factor of 100. Following the integration of the novel PCR into the Hessian wildlife-monitoring program, CAdV-1 DNA was detected in none of the tested raccoons ( n = 48) but in 11 of 97 foxes.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Ratons laveurs
/
Adénovirus canins
/
Réaction de polymérisation en chaine en temps réel
/
Renards
Type d'étude:
Diagnostic_studies
Limites:
Animals
Pays/Région comme sujet:
Europa
Langue:
En
Journal:
J Vet Diagn Invest
Sujet du journal:
MEDICINA VETERINARIA
Année:
2017
Type de document:
Article
Pays d'affiliation:
Allemagne