Genome-Wide Analysis of DNA Methylation in Hematopoietic Cells: DNA Methylation Analysis by WGBS.
Methods Mol Biol
; 1633: 137-149, 2017.
Article
de En
| MEDLINE
| ID: mdl-28735485
ABSTRACT
DNA methylation is a major epigenetic modification that regulates gene expression, genome imprinting, and development and has a role in diseases including cancer. There are various methods for whole-genome methylation profiling that differ in cost and resolution. Recent advances in high-throughput sequencing technologies coupled with bisulfite treatment enable absolute DNA methylation quantification and genome-wide single-nucleotide resolution analysis. In this chapter, we provide detailed protocols for whole-genome bisulfite sequencing (WGBS), which captures the complete methylome. Using WGBS, we are able to generate a reference DNA methylome for normal or malignant hematopoietic cells.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
ADN tumoral
/
Leucémie aigüe myéloïde
/
Génome humain
/
Méthylation de l'ADN
/
Séquençage nucléotidique à haut débit
/
Séquençage du génome entier
Type d'étude:
Guideline
Limites:
Humans
Langue:
En
Journal:
Methods Mol Biol
Sujet du journal:
BIOLOGIA MOLECULAR
Année:
2017
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique