Structured illumination microscopy reveals actin I localization in discreet foci in Plasmodium berghei gametocytes.
Exp Parasitol
; 181: 82-87, 2017 Oct.
Article
de En
| MEDLINE
| ID: mdl-28803903
ABSTRACT
Actin has important roles in Plasmodium parasites but its exact function in different life stages is not yet fully elucidated. Here we report the localization of ubiquitous actin I in gametocytes of the rodent model parasite P. berghei. Using an antibody specifically recognizing F-actin and deconvolution microscopy we detected actin I in a punctate pattern in gametocytes. 3D-Structured Illumination Microscopy which allows sub-diffraction limit imaging resolved the signal into structures of less than 130 nm length. A portion of actin I was soluble, but the protein was also found complexed in a stabilized form which could only be completely solubilized by treatment with SDS. An additional population of actin was pelleted at 100 000 × g, consistent with F-actin. Our results suggest that actin in this non-motile form of the parasite is present in short filaments cross-linked to other structures in a cytoskeleton.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Plasmodium berghei
/
Actines
Limites:
Animals
Langue:
En
Journal:
Exp Parasitol
Année:
2017
Type de document:
Article
Pays d'affiliation:
Grèce