Deconstruction of the beaten Path-Sidestep interaction network provides insights into neuromuscular system development.
Elife
; 62017 08 15.
Article
de En
| MEDLINE
| ID: mdl-28829740
ABSTRACT
An 'interactome' screen of all Drosophila cell-surface and secreted proteins containing immunoglobulin superfamily (IgSF) domains discovered a network formed by paralogs of Beaten Path (Beat) and Sidestep (Side), a ligand-receptor pair that is central to motor axon guidance. Here we describe a new method for interactome screening, the Bio-Plex Interactome Assay (BPIA), which allows identification of many interactions in a single sample. Using the BPIA, we 'deorphanized' four more members of the Beat-Side network. We confirmed interactions using surface plasmon resonance. The expression patterns of beat and side genes suggest that Beats are neuronal receptors for Sides expressed on peripheral tissues. side-VI is expressed in muscle fibers targeted by the ISNb nerve, as well as at growth cone choice points and synaptic targets for the ISN and TN nerves. beat-V genes, encoding Side-VI receptors, are expressed in ISNb and ISN motor neurons.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Cônes de croissance
/
Protéines de Drosophila
/
Drosophila melanogaster
/
Protéines membranaires
/
Motoneurones
/
Muscles
/
Protéines de tissu nerveux
/
Système nerveux
Limites:
Animals
Langue:
En
Journal:
Elife
Année:
2017
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique