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Proteomic Analysis of Secreted Proteins from Cell Microenvironment.
Adhikari, Subash; Chen, Lan; Huang, Peiwu; Tian, Ruijun.
Affiliation
  • Adhikari S; Department of Chemistry, Southern University of Science and Technology, 1088 Xueyuan Rd., Nanshan District, Guangdong, Shenzhen, 518055, People's Republic of China.
  • Chen L; Department of Chemistry, Southern University of Science and Technology, 1088 Xueyuan Rd., Nanshan District, Guangdong, Shenzhen, 518055, People's Republic of China.
  • Huang P; Department of Chemistry, Southern University of Science and Technology, 1088 Xueyuan Rd., Nanshan District, Guangdong, Shenzhen, 518055, People's Republic of China.
  • Tian R; Department of Chemistry, Southern University of Science and Technology, 1088 Xueyuan Rd., Nanshan District, Guangdong, Shenzhen, 518055, People's Republic of China. tian.rj@sustc.edu.cn.
Methods Mol Biol ; 1662: 45-58, 2017.
Article de En | MEDLINE | ID: mdl-28861816
ABSTRACT
Cell microenvironment consists of various types of cells which communicate with each other by vast number of secreted proteins. An unbiased profiling of these secreted proteins on a global scale is often critical for understanding the intercellular signaling in an autocrine or paracrine manner. Mass spectrometry-based proteomics has become one of the most popular technology for characterization of the secreted proteins. In this chapter, we discuss the standard workflow for secreted proteins characterization, including harvesting secreted proteins from conditioned media, digesting the obtained proteins, liquid chromatography-mass spectrometry analysis, and downstream data analysis.
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Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Régulation de l'expression des gènes tumoraux / Chromatographie en phase liquide / Protéome / Spectrométrie de masse en tandem / Microenvironnement tumoral / Protéines tumorales Limites: Humans Langue: En Journal: Methods Mol Biol Sujet du journal: BIOLOGIA MOLECULAR Année: 2017 Type de document: Article
Texte intégral
Ajouter à My VHL
Imprimer
XML
PubMed Links
Recherche sur Google

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Régulation de l'expression des gènes tumoraux / Chromatographie en phase liquide / Protéome / Spectrométrie de masse en tandem / Microenvironnement tumoral / Protéines tumorales Limites: Humans Langue: En Journal: Methods Mol Biol Sujet du journal: BIOLOGIA MOLECULAR Année: 2017 Type de document: Article