T-bet, but not Gata3, overexpression is detrimental in a neurotropic viral infection.
Sci Rep
; 7(1): 10496, 2017 09 05.
Article
de En
| MEDLINE
| ID: mdl-28874814
ABSTRACT
Intracerebral Theiler's murine encephalomyelitis virus (TMEV) infection in mice induces inflammatory demyelination in the central nervous system. Although C57BL/6 mice normally resistant to TMEV infection with viral clearance, we have previously demonstrated that RORγt-transgenic (tg) C57BL/6 mice, which have Th17-biased responses due to RORγt overexpression in T cells, became susceptible to TMEV infection with viral persistence. Here, using T-bet-tg C57BL/6 mice and Gata3-tg C57BL/6 mice, we demonstrated that overexpression of T-bet, but not Gata3, in T cells was detrimental in TMEV infection. Unexpectedly, T-bet-tg mice died 2 to 3 weeks after infection due to failure of viral clearance. Here, TMEV infection induced splenic T cell depletion, which was associated with lower anti-viral antibody and T cell responses. In contrast, Gata3-tg mice remained resistant, while Gata3-tg mice had lower IFN-γ and higher IL-4 production with increased anti-viral IgG1 responses. Thus, our data identify how overexpression of T-bet and Gata3 in T cells alters anti-viral immunity and confers susceptibility to TMEV infection.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Poliomyélite
/
Expression des gènes
/
Theilovirus
/
Protéines à domaine boîte-T
/
Facteur de transcription GATA-3
Type d'étude:
Prognostic_studies
Limites:
Animals
Langue:
En
Journal:
Sci Rep
Année:
2017
Type de document:
Article
Pays d'affiliation:
Japon