Evaluation of FASP, SP3, and iST Protocols for Proteomic Sample Preparation in the Low Microgram Range.

Sielaff, Malte; Kuharev, Jörg; Bohn, Toszka; Hahlbrock, Jennifer; Bopp, Tobias; Tenzer, Stefan; Distler, Ute

Sielaff, Malte; Kuharev, Jörg; Bohn, Toszka; Hahlbrock, Jennifer; Bopp, Tobias; Tenzer, Stefan; Distler, Ute.

Affiliation

Sielaff M; Institute for Immunology, University Medical Center of the Johannes Gutenberg University Mainz , Langenbeckstr. 1, 55131 Mainz, Germany.
Kuharev J; Institute for Immunology, University Medical Center of the Johannes Gutenberg University Mainz , Langenbeckstr. 1, 55131 Mainz, Germany.
Bohn T; Institute for Immunology, University Medical Center of the Johannes Gutenberg University Mainz , Langenbeckstr. 1, 55131 Mainz, Germany.
Hahlbrock J; Institute for Immunology, University Medical Center of the Johannes Gutenberg University Mainz , Langenbeckstr. 1, 55131 Mainz, Germany.
Bopp T; Institute for Immunology, University Medical Center of the Johannes Gutenberg University Mainz , Langenbeckstr. 1, 55131 Mainz, Germany.
Tenzer S; Institute for Immunology, University Medical Center of the Johannes Gutenberg University Mainz , Langenbeckstr. 1, 55131 Mainz, Germany.
Distler U; Institute for Immunology, University Medical Center of the Johannes Gutenberg University Mainz , Langenbeckstr. 1, 55131 Mainz, Germany.

J Proteome Res ; 16(11): 4060-4072, 2017 11 03.

Article de En | MEDLINE | ID: mdl-28948796

Sujet(s)
Mots clés

bottom-up proteomics; filter-aided sample preparation (FASP); in-StageTip digestion (iST); sample preparation; single-pot solid-phase-enhanced sample preparation (SP3); tumor-associated macrophages (TAMs)

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Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Manipulation d'échantillons / Protéomique Type d'étude: Evaluation_studies / Prognostic_studies Limites: Humans Langue: En Journal: J Proteome Res Sujet du journal: BIOQUIMICA Année: 2017 Type de document: Article Pays d'affiliation: Allemagne Pays de publication: États-Unis d'Amérique

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