A complex genomic locus drives mtDNA replicase POLG expression to its disease-related nervous system regions.
EMBO Mol Med
; 10(1): 13-21, 2018 01.
Article
de En
| MEDLINE
| ID: mdl-29109127
ABSTRACT
DNA polymerase gamma (POLG), the mtDNA replicase, is a common cause of mitochondrial neurodegeneration. Why POLG defects especially cause central nervous system (CNS) diseases is unknown. We discovered a complex genomic regulatory locus for POLG, containing three functional CNS-specific enhancers that drive expression specifically in oculomotor complex and sensory interneurons of the spinal cord, completely overlapping with the regions showing neuronal death in POLG patients. The regulatory locus also expresses two functional RNAs, LINC00925-RNA and MIR9-3, which are coexpressed with POLG The MIR9-3 targets include NR2E1, a transcription factor maintaining neural stem cells in undifferentiated state, and MTHFD2, the regulatory enzyme of mitochondrial folate cycle, linking POLG expression to stem cell differentiation and folate metabolism. Our evidence suggests that distant genomic non-coding regions contribute to regulation of genes encoding mitochondrial proteins. Such genomic arrangement of POLG locus, driving expression to CNS regions affected in POLG patients, presents a potential mechanism for CNS-specific manifestations in POLG disease.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
ADN mitochondrial
/
Maladies du système nerveux central
/
Régulation de l'expression des gènes
/
Locus génétiques
/
DNA Polymerase gamma
Limites:
Animals
/
Humans
/
Male
Langue:
En
Journal:
EMBO Mol Med
Sujet du journal:
BIOLOGIA MOLECULAR
Année:
2018
Type de document:
Article
Pays d'affiliation:
Finlande