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Laser surface modification of decellularized extracellular cartilage matrix for cartilage tissue engineering.
Goldberg-Bockhorn, Eva; Schwarz, Silke; Subedi, Rachana; Elsässer, Alexander; Riepl, Ricarda; Walther, Paul; Körber, Ludwig; Breiter, Roman; Stock, Karl; Rotter, Nicole.
Affiliation
  • Goldberg-Bockhorn E; Department of Otorhinolaryngology, Head and Neck Surgery, Ulm University Medical Center, Frauensteige 12, 89075, Ulm, Germany. eva.goldberg@uniklinik-ulm.de.
  • Schwarz S; Department of Anatomy, Paracelsus Medical University, Salzburg and Nuernberg, Prof. Ernst Nathan Str. 1, 90419, Nuernberg, Germany.
  • Subedi R; Department of Otorhinolaryngology, Head and Neck Surgery, Ulm University Medical Center, Frauensteige 12, 89075, Ulm, Germany.
  • Elsässer A; Department of Otorhinolaryngology, Head and Neck Surgery, Ulm University Medical Center, Frauensteige 12, 89075, Ulm, Germany.
  • Riepl R; Department of Otorhinolaryngology, Head and Neck Surgery, Ulm University Medical Center, Frauensteige 12, 89075, Ulm, Germany.
  • Walther P; Central Facility for Electron Microscopy, Ulm University, Albert-Einstein-Allee 11, 89081, Ulm, Germany.
  • Körber L; Institute of Bioprocess Engineering, Erlangen University, Paul-Gordan-Str. 3, 91052, Erlangen, Germany.
  • Breiter R; Chair of Medical Bio-Technology, University of Erlangen, Paul-Gordan-Str. 3, 91052, Erlangen, Germany.
  • Stock K; Institut für Lasertechnologien in der Medizin und Meßtechnik, an der Universität Ulm, Helmholtzstr. 12, 89081, Ulm, Germany.
  • Rotter N; Department of Otorhinolaryngology, Mannheim University Medical Center, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany.
Lasers Med Sci ; 33(2): 375-384, 2018 Feb.
Article de En | MEDLINE | ID: mdl-29209868
ABSTRACT
The implantation of autologous cartilage as the gold standard operative procedure for the reconstruction of cartilage defects in the head and neck region unfortunately implicates a variety of negative effects at the donor site. Tissue-engineered cartilage appears to be a promising alternative. However, due to the complex requirements, the optimal material is yet to be determined. As demonstrated previously, decellularized porcine cartilage (DECM) might be a good option to engineer vital cartilage. As the dense structure of DECM limits cellular infiltration, we investigated surface modifications of the scaffolds by carbon dioxide (CO2) and ErYAG laser application to facilitate the migration of chondrocytes inside the scaffold. After laser treatment, the scaffolds were seeded with human nasal septal chondrocytes and analyzed with respect to cell migration and formation of new extracellular matrix proteins. Histology, immunohistochemistry, SEM, and TEM examination revealed an increase of the scaffolds' surface area with proliferation of cell numbers on the scaffolds for both laser types. The lack of cytotoxic effects was demonstrated by standard cytotoxicity testing. However, a thermal denaturation area seemed to hinder the migration of the chondrocytes inside the scaffolds, even more so after CO2 laser treatment. Therefore, the ErYAG laser seemed to be better suitable. Further modifications of the laser adjustments or the use of alternative laser systems might be advantageous for surface enlargement and to facilitate migration of chondrocytes into the scaffold in one step.
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Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Cartilage / Ingénierie tissulaire / Matrice extracellulaire / Lasers à semiconducteur Limites: Adult / Animals / Humans Langue: En Journal: Lasers Med Sci Sujet du journal: BIOTECNOLOGIA / RADIOLOGIA Année: 2018 Type de document: Article Pays d'affiliation: Allemagne

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Cartilage / Ingénierie tissulaire / Matrice extracellulaire / Lasers à semiconducteur Limites: Adult / Animals / Humans Langue: En Journal: Lasers Med Sci Sujet du journal: BIOTECNOLOGIA / RADIOLOGIA Année: 2018 Type de document: Article Pays d'affiliation: Allemagne